Instantaneous determination of antihypertensive drugs utilizing TLC: Densitometric methods
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Nahda University, New Beni-Swif, Egypt.
Research Article
International Journal of Frontiers in Life Science Research, 2022, 02(02), 026–036.
Article DOI: 10.53294/ijflsr.2022.2.2.0022
Publication history:
Received on 05 January 2022; revised on 09 April 2022; accepted on 11 April 2022
Abstract:
An accurate, sensitive and time saving thin layer chromatography (TLC) - densitometric method has been developed and validated for determination of two Antihypertensive drug mixtures. Mixture one contains Spironolactone, Furosemide and Anthranilic acid while, mixture two contains Triamterene, Hydrochlorothiazide and chlorothiazide. Mixture 1 uses developing system consist of Hexane, Ethyl acetate and Glacial acetic acid (5ml – 5ml – 0.1ml ) as developing system and 240 nm as scanning wavelength for separation and determination of Spironolactone, Furosemide and Anthranilic acid (metabolite of Furosemide) and mixture 2 uses developing system consist of Ethyl acetate, Methanol, Glacial acetic acid and Ammonium hydroxide (8ml - 1ml - 0.2ml - 0.3 ml) as developing system and 300nm as scanning wave length for separation and determination of Triamterene, Hydrochlorothiazide and Chlorothiazide ( impurity of Hydrochlorothiazide).
This method was validated and shown to demonstrate good accuracy and precision according to the International Council for Harmonisation (ICH) guidelines.
Keywords:
Anthranilic acid; Chlorothiazide; Furosemide; Hydrochlorothiazide; Spironolactone; Triamterene; TLC Densitometry
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