Structural determination of an isolated vasodilatory molecule from Ludwigia octovalvis (Jacq.) P.H. Raven (Onagraceae)
1 Department of Pharmacognosy and Herbal Medicine, University of Ghana, Box LG 610, Legon Accra, Ghana.
2 Department of Agronomy and Agri-Food, Engineering School of Agronomy and Environment, Higher Institute of Technology of Ambositra, BP 306, Madagascar.
3 Department of Chemistry, Sciences Faculty, BP 1246-301, University of Fianarantsoa, Madagascar.
4 Department of Pharmacology, Sciences Faculty, BP 906, University of Antananarivo, Madagascar.
Research Article
International Journal of Frontiers in Chemistry and Pharmacy Research, 2023, 03(02), 008–015.
Article DOI: 10.53294/ijfcpr.2023.3.2.0060
Publication history:
Received on 09 September 2023; revised on 28 November 2023; accepted on 01 December 2023
Abstract:
The determination of the chemical structure of an isolated vasodilatory molecule from Ludwigia octovalvis (Jacq.) P.H. Raven (Onagraceae) was undertaken. This molecule was isolated from n-butanolic extract using bioassay-guided isolation with preparative plate chromatographic techniques and its structure was determined using spectroscopy (UV, 1D- and 2D NMR, MS). The vasodilatory activity of the isolate was investigated on guinea pig isolated aorta. The results show 3 spots from n-butanolic extract with respective retention factor (Rf) of 0.14, 0.6 and 0.91. Pharmacological investigations showed that the isolated molecule with Rf 0.91 relaxes the isolated aorta pre-contracted by norepinephrine. Data from chemical shifts (δH) and spin-spin coupling constants (JHH) were compared with literature to confirm the isolate as rutin. This is the first report of rutin isolated from Ludwigia octovalvis.
Keywords:
Ludwigia octovalvis; Vasodilatory; n-Butanolic; Rutin
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